high-dha diet  (Bio-Serv)

Journal: Aging Cell

Article Title: Deuterated docosahexaenoic acid protects against oxidative stress and geographic atrophy‐like retinal degeneration in a mouse model with iron overload

doi: 10.1111/acel.13579

Figure Lengend Snippet: D‐DHA content as a percentage of D‐DHA +DHA in neural retina and RPE from mice fed with D‐DHA or DHA at 4 weeks, given IVT injections, and then continued on the D‐DHA diet for another week

Article Snippet: To determine ocular D‐DHA accretion, the mice were switched from the laboratory rodent diet to the experimental D‐DHA supplemented diet containing 0.5% D‐DHA plus 6.5% high oleic soybean oil (w/w) in AIN93G (Reeves et al., ) (Research Diet, Inc.).

Techniques: Saline

Journal: Translational Psychiatry

Article Title: Convergent functional genomic studies of omega-3 fatty acids in stress reactivity, bipolar disorder and alcoholism

doi: 10.1038/tp.2011.1

Figure Lengend Snippet: Effects of docosahexaenoic acid (DHA) on male DBP knockout (KO) stressed (ST) mice alcohol (EtOH) consumption: mice on a diet supplemented with either low or high DHA were subjected to alcohol free-choice drinking paradigm. ( a , b ) Fluid consumption (water or 10% ethanol) monitored for a period of 2 weeks (14 days). ( c , d ) Fluid consumption (water or 10% ethanol) monitored for a period of 4 weeks (28 days) with an acute stressor (behavioral challenge tests represented by the dotted vertical line) at day 21, as described in the Materials and methods Section. * P <0.05.

Article Snippet: All mice were housed for at least 1 week before each experiment in a room set to an alternating light cycle with 12 h of darkness from 1000 to 2200 h, and 12 h of light from 2200 to 1000 h. At the start of the experiment, male and female DBP (+/+) WT or DBP (−/−) KO mice were placed on one of the two diets: (1) low DHA custom research diet (TD 00522, Harlan Teklad, Madison, WI, USA), a DHA-depleting low n-3 PUFA test diet adequate in all other nutrients (n-6/ n- 3 ratio of 85:1 with 6% fat as safflower oil); or (2) high DHA custom research diet (TD 07708 low-DHA diet supplemented with 0.69% algal DHA; Martek Bioscience, Columbia, MD, USA).

Techniques: Knock-Out

Journal: Translational Psychiatry

Article Title: Convergent functional genomic studies of omega-3 fatty acids in stress reactivity, bipolar disorder and alcoholism

doi: 10.1038/tp.2011.1

Figure Lengend Snippet: Effects of docosahexaenoic acid (DHA) on alcohol (EtOH) consumption in male alcohol-preferring (P) rats. Experimentally naive, male P rats, 4–6 months of age at the start of the experiment, were used as subjects. These rats were placed on one of the three diets: (1) low-DHA diet, (2) control diet or (3) high-DHA diet. Rats were given continuous free-choice access in the home cage to 15% v/v ethanol and water. Ethanol intake was measured daily throughout the experiment. ( a , b ) Fluid consumption from both bottles was monitored for a period of 2 weeks (14 days). * t -test P <0.05 for rats on low-DHA compared with rats on high-DHA diet.

Article Snippet: All mice were housed for at least 1 week before each experiment in a room set to an alternating light cycle with 12 h of darkness from 1000 to 2200 h, and 12 h of light from 2200 to 1000 h. At the start of the experiment, male and female DBP (+/+) WT or DBP (−/−) KO mice were placed on one of the two diets: (1) low DHA custom research diet (TD 00522, Harlan Teklad, Madison, WI, USA), a DHA-depleting low n-3 PUFA test diet adequate in all other nutrients (n-6/ n- 3 ratio of 85:1 with 6% fat as safflower oil); or (2) high DHA custom research diet (TD 07708 low-DHA diet supplemented with 0.69% algal DHA; Martek Bioscience, Columbia, MD, USA).

Techniques: Control